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范恩国教授
[ 作者:基础医学研究所(院)    来源自:本站原创    点击数:309    更新时间:2017-9-12    文章编辑:wangluo ]

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范恩国,男,博士。2008年获德国马丁-路德大学博士学位。2008年-2010 年,在德国弗莱堡大学医学院进行博士后研究; 2010年-2017年历任弗莱堡大学医学院助理研究员/课题组长及研究员(PI),期间参与或主持多项德国国家(DFG)课题,总经费近200万欧元,并与德国、法国、挪威、奥地利、瑞典、日本等国以及国内的多个课题组开展了密切合作。已培养博士1人(Summa Cum Laude毕业),医学博士1人,硕士数人并指导博士后2人;2017年3月起,任中国医学科学院/北京协和医学院“协和学者”特聘教授,任职于病原生物学系。

研究方向 
    鉴于革兰氏阴性菌外膜上的β-桶状蛋白在细菌的免疫原性、致病性和耐药性方面的重要作用,以及其位于阴性菌的最外层因而更易为相关药物识别并结合的特点,因此外膜桶状蛋白是非常理想的新型抗生素作用靶点。故本课题组的研究方向为膜蛋白的生成与演化以及蛋白质的跨膜转运机理,具体为革兰氏阴性菌外膜β-桶状蛋白的膜整合与演化和病原蛋白跨细菌外膜的转运机理研究; 以外膜蛋白为靶点的新型抗生素开发(包括天然产物的分离分析及基于此的药物筛选) ; 基于蛋白质跨细菌外膜转运的细菌细胞表面蛋白展示技术为基础的生物工程产品开发(如疫苗开发等)。
    拟使用的研究方法包括蛋白质跨膜转运离体重组系统(versatile in vitro reconstitution system for beta-barrel outer membrane proteins)、蛋白质定点光交联技术(site-specific photo-cross-linking)、蛋白质的离体合成技术(in vitro protein transcription/translation system)及相关的分子生物学、生物化学、生物工程及分析化学与化工、天然产物化学等。
    目前课题组有1名博士研究生,1名硕士研究生,拟聘助研(具博士学位)1-2名,技术员1名,有意者请与本课题组联系。


联系方式:
中国医学科学院/北京协和医学院
基础医学研究所病原生物学系老科研楼544室
北京市东城区东单三条5号


邮编: 100005
电话:010-69156984
电子邮件:enguo.fan@ibms.pumc.edu.cn


近5年发表的论文: 


*通讯作者
1,Chuanyi Yao*, Jinliang Chen, Yinghua Lu, Shaokun Tang, Enguo Fan*. Construction of an asynchronous three-zone simulated-moving-bed chromatography and its application for the separation of vanillin and syringaldehyde, Chemical Engineering Journal,2017 (In press, DOI: doi.org/10.1016/j.cej.2017.09.006).
2,Shanshan Tian, Shuzhen Zheng, Yanpu Han, Zhenchang Guo, Guijin Zhai, Xue Bai, Xi-Wen He, Enguo Fan, YuKui Zhang, and Kai Zhang. Maleic Anhydride Labeling-based Approach for Quantitative Proteomics and Successive Derivatization of Peptides. Analytical Chemistry, 2017, 89(16):8259-8265.
3, Wenjing Bi, Xue Bai, Fan Gao, Congcong Lu, Ye Wang, Guijin Zhai, Shanshan Tian, Enguo Fan. Yukui Zhang, and Kai Zhang, DNA-Templated Aptamer Probe for Identification of Target Proteins. Analytical Chemistry, 2017, 89(7):4071-4076.
4, Chuanyi Yao*, Kaili Yue, Xueping Ling, Keju Jing, Yinghua Lu, Shaokun Tang and Enguo Fan*. Biocatalytic synthesis of vitamin A palmitate using immobilized lipase produced by recombinant Pichia pastoris, Engineering in Life Sciences, 2017, 17: 768-774.
5, Chengchao Ding, Enguo Fan, Shujuan Wang, Liang Guo, Jie Li, Qing Liu. A potential aquaculture vaccine vector: Evaluation of a double-gene attenuated Listeria monocytogenes in zebrafish ( Danio rerio ), Aquaculture, 2017, 479: 311-320.
6, Dongmei Xi, Jizhen Shang, Enguo Fan, Jinmao You, Shusheng Zhang, and Hua Wang, Nanopore-Based Selective Discrimination of MicroRNAs with Single-Nucleotide Difference Using Locked Nucleic Acid-Modified Probes, Analytical Chemistry, 2016, 88(21): 10540-10546.
7, Xue Bai, Congcong Lu, Jin Jin, Shanshan Tian, Zhenchang Guo, Pu Chen, Guijin Zhai, Shuzhen Zheng, Xiwen He, Enguo Fan, Yukui Zhang, and Kai Zhang. Development of DNA-templated Peptide Probe for Photo-affinity Labeling and Enrichment of the 'Readers' of Histone Modification,Angewandte Chemie International Edition, 2016, 55(28): 7993-7997.
8, Haijuan Zeng, Wenbo Guo, Beibei Liang, Jianwu Li, Xuzhao Zhai, Chunmei Song,   Wenjun Zhao*, Enguo Fan*, Qing Liu*, Self-Paired Monoclonal Antibody Lateral Flow Immunoassay Strip for Rapid Detection of Acidovorax avenae subsp. Citrulli, Analytical and Bioanalytical Chemistry, 2016, 408:6071–6078.
9, Enguo Fan, Nandini Chauhan, Gupta Udatha, Jack Leo, Dirk Linke. Type V secretion systems in bacteria, Microbiology Spectrum, 2016, 3(6): VMBF-0009-2015. in Virulence Mechanisms of Bacterial Pathogens, Fifth Edition, ASM press (Book chapter).
10, Enguo Fan, Derrick Norell, Matthias Müller. An In Vitro Assay for Substrate Translocation by FhaC in Liposomes. Methods in Molecular Biology, 2015, 1329:111-125.
11, Luisa Estrada Mallarino, Enguo Fan, Meike Odermatt, Matthias Müller, Jie Liang, MeiShan Lin, Martin Heinzelmann, Fenja Fritsche, Hans-Jürgen Apell, Wolfram Welte. TtOmp85, a β-Barrel Assembly Protein, Functions by Barrel Augmentation. Biochemistry, 2015, 54 (3): 844–852.
12, Yanfang Wu, Xinsheng Wang, Enguo Fan*, Extraction, Structure, and Bioactivities of the Polysaccharides from Fructus. Book chapter in Polysaccharides: Bioactivity and Biotechnology (Edited by Jean-Michel Merillon and Kishan G. Ramawat), Springer Verlag, Germany, 2015, 1585-1607 (Book chapter).
13, Man Wu,   Haolin Li,   Yinghui Jiang,   Chunmei Song,   Huiqin Guo,   Shuyan Wu,   Cheng Liu,  Jianwu Li,  Haijuan Zeng,  Xuzhao Zhai,  Weisheng Zhang,*  Enguo Fan* and  Qing Liu*. Simultaneous quantification of Escherichia coli O157:H7 and Shigella boydii using a visual-antibody-macroarray. Analyst, 2015, 140(19):6595-601.
14, Ye Wang, Yanpu Han, Enguo Fan*, Kai Zhang*. Analytical strategies used to identify the readers of histone modifications: A review. Analytica Chimica Acta, 2015, 891:32-42.
15, Yanpu Han, Congcong Lu, Kai Zhang, Shanshan Tian, Enguo Fan, Langxing Chen, Xi-Wen He and Yukui Zhang. Quantitative characterization of histones post-translational modifications using stable isotope dimethyl-labeling strategy. Analytical Methods, 2015, 7: 3779-3785.
16, Derrick Norell, Alexander Heuck, Thuy-Anh Tran-Thi, Hansjörg Götzke, Françoise Jacob-Dubuisson, Tim Clausen, Daniel O. Daley, Volkmar Braun, Matthias Müller*, Enguo Fan*, Versatile in vitro Assay for Bacterial Outer Membrane Protein Translocation and Integration Analysis. Nature Communications, 2014, 5:5396.
17, Kai Zhang, Liyan Li, Mengxiao Zhu, Guojuan Wang, Jianjun Xie, Yunlong Zhao, Enguo Fan, Liyan Xu, Enmin Li, Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities. Journal of Proteomics, 2014, 2014, 112C:180-189.
18, Qiang Wang, Xin Zhao, Yanrong Ren, Enguo Fan, Haijun Chang, Hongbin Wu, Effects of high pressure treatment and temperature on lipid oxidation and fatty acid composition of yak (Poephagus grunniens) body fat. Meat Science. 2013, 94(4):489-494.
19, Changzhen Wang, Hongmei Zhou, Ruiyun Peng, Lifeng Wang, Zhentao Su, Peng Chen, Shuiming Wang, Shaoxia Wang, Yao Liu, Jianbo Cong, Ke Wu*, Xiangjun Hu*, Enguo Fan*, Electromagnetic Pulse Reduces Free Radical Generation in Rat Liver Mitochondria In Vitro, Free Radical Research 2013, 47(4): 276-82.
20, Kai Zhang*, Shanshan Tian, Enguo Fan*, Protein lysine acetylation analysis: current MS-based proteomic technologies. Analyst. 2013, 138 (6), 1628-1636.
21, Yanfang Wu, Xinsheng Wang, Biao Shen, Lei Kang, Enguo Fan*, Extraction of polysaccharides from plant. Recent Patents on Food, Nutrition & Agriculture, 2013, 5: 57-61.
22, Enguo Fan, Silke Fiedler, Francoise Jacob-Dubuisson, Matthias Müller, Two-partner secretion of gram-negative bacteria: A single β-barrel protein enables transport across the outer membrane, Journal of Biological Chemistry, 2012, 287(4): 2591-2599.
23, Yanfang Wu, Xinsheng Wang, Enguo Fan*, Optimization of ultrasound-assisted extraction of puerarin and total isoflavone from puerariae lobatae radix (Pueraria lobata (Wild.) Ohwi) with response surface methodology, Phytochemical Analysis, 2012, 23: 513–519.

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